flowjo compensation matrix negative values

#Flowjo compensation matrix negative values software

This will add a small random value to the linear value prior to it being reconverted back to log. Typically, this value should be less than one. compensated flow cytometry data that frequently contain negative values. The compensation matrix is represented by the grid to the left of the sample name. The positive should be at least as bright as anything that will be encountered in the experiment and should form at least 10 of the population. Compensation Matrix values stored within the FCS header. Create new, apply old, or compare different matrices. These controls should be solely used to set compensation. However, if you notice digitization errors, you can enter a value to spread the grouped population out using the Range of random value to add when converting log data field (Figure 9.11). Compensation controls are required for each fluorochrome and should contain both a positive and a negative population.

#Flowjo compensation matrix negative values software

The conversion to linear and reconversion back to log occurs automatically during the software compensation process. Depending on the resolution of your original file, this can result in digitization errors, which can cause large numbers of events to group in a single channel. It is then converted back to log for display. If you have stored data on a logarithmic scale in your FCS file (common with FCS 2.0 data files), this data has to be converted to a linear scale before compensation is applied. You may find it helpful to enter the date or experiment number as the compensation name.Ĭompensation must be performed on linear data. 1.48K subscribers Subscribe 1.5K views 1 year ago Are you new to data analysis in FlowJo or looking for a starting point This video will give you a quick overview on compensating your flow. The name of your compensation may be changed at any time by selecting the compensation and clicking the Rename selected compensation button or by right-clicking on a compensation and choosing Rename. When a compensation value is manually adjusted, the compensation icon (red box at the top) becomes red highlighted, while the text value (red box at the bottom) becomes bold and underlined.Įvery compensation must be given a unique name.